Objective : In order to determine the prevention effects of oral administration with HyeungBungJiHumflangW3JHT) on the liver cirrhosis of rats induced by long time carbon tetrachloride injection, the changes of body weight and gains, absolute and relative liver weight, biochemistry of blood, histopathological changes, urine specific gravity and urine bilirubin levels were monitored after 10 weeks of concomitantly dosing of HBJHT extract(per os) and CCl₄(i.p).
Method : The WBJHT extracts were daily dosed at 50(T1), l00(T2) and 200(T3)ms/kg for 10 weeks. Result : The body weight and gains were significantly and dose dependently increased in HBJHT treated group compared to that of CCl₄treated control group. The absolute and relative liver weight were significantly and dose dependently reduced in HBJHT treated group compared to that of CCl₄treated control group. Severe hepatocellular necrosis and ballooning, hyperplasia of connective tissue and subdue reduction of hepatic lobule, hyperplasia of bile duct in portal triad were dramatically decreased in HBJHT treated group compared to that of CCl₄treated control group in histopathological observation.The diameter of hepatic lobules was significantly enlarged, and in HBJHT treated group compared to that of cc14 treated control group, and the numbers of connective tissue, degenerative cell and bile duct were significantly and dose dependently decreased. The levels of serum AST, ALT, LDH, ALP, GGT and total bilirubin were SignificantJy and dose dependently reduced in HBJHT treated group compared to that of CCl₄treated control group. The urine specific gravities were significantly and dose dependently increased, and the urine bilirubin levels were decreased in HBJHT treated group compared to that of CCl₄treated control group.
Conclustions : it is considered that HBJWT extracts have some favorable effect to prevent the induce of hepatic cirrhosis by CCl₄injection.
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